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Polyclonal anti-N-cadherin (Cat.# M142) Polyclonal anti-P, E, N-cadherin (Cat.# M143) Polyclonal anti Pan cadherin (Sigma C-3678) A: B: C: D
Add 100 μl of prepared 1x rabbit anti-phospho-Akt (Ser473) antibody or 1x rabbit anti-pan-Akt (Reagent Preparation step 5) to appropriate wells.
Do this calculation separately for the anti-pan-protein specific antibody and secondary antibody using their dilution factors and the total volumes in the table above.
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