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... of a 0.10 µM solution of memory DNA in 1×SSC (150 mM sodium chloride and 15 mM sodium citrate) was measured by using a fluorescence spectrometer LS55 (Perkin-Elmer, MA, USA).
After shaking at room temperature for 5min, the chemiluminescence was measured using a Wallac 1420 ARVO SX multi-label counter (Perkin-Elmer, MA, U.S.A.). hAR Mediated Reporter Gene ...
A thermal cycler (GeneAmp PCR system 2400, Per-kin Elmer, MA, U.S.A.) was used with the following specifications: 94 ℃ for 6 min; 35 cycles of 94 ℃ for 1 min, 62 ℃ for ...
The resulting cDNA was used as a template for PCR amplification using Amplitaq polymerase (Perkin-Elmer, MA) and 5' and 3' primers of Ig-prime kit (Novagen, Darmstadt, Germany ...
... were performed in 5 μl volumes, and included 100 ng of template (genomic DNA), each primer at 10 pmol/μl, 2.5 mM dNTP, and 1 U of Taq polymerase (Perkin-Elmer, MA, USA) in ...
... of a 0.10 µM solution of memory DNA in 1×SSC (150 mM sodium chloride and 15 mM sodium citrate) was measured by using a fluorescence spectrometer LS55 (Perkin-Elmer, MA, ...
After shaking at room temperature for 5min, the chemiluminescence was measured using a Wallac 1420 ARVO SX multi-label counter (Perkin-Elmer, MA, U.S.A.). hAR Mediated ...
A thermal cycler (GeneAmp PCR system 2400, Per-kin Elmer, MA, U.S.A.) was used with the following specifications: 94 for 6 min; 35 cycles of 94 for 1 min ...
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