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1.5M Tris-Cl (pH 8.8) 8x Stacking-gel buffer: 1M Tris-Cl (pH 6.8) 1x SDS gel-loading buffer: 50mM Tris-Cl (pH 6.8) 100mM dithiothreitol: 2% SDS: 0.1% bromophenol blue
10 mM Tris-Cl (pH 8.0) 0.2 mM EDTA. Note: We routinely use A18 primer and pTZrev primer shown below for colony PCR. The nucleotide sequence of the figure below is from pUC118
DFT Calculations on Manganese(III)5,10,15-Tris(pentafluorophenyl)-Corrole. LIU Hai-Yang; LI Li; YING Xiao; WANG Xiang-Li; XU Zhi-Guang; LIAO Shi-Jun; CHANG Chi-Kwong
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