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50 mM Tris HCl. What is the Tris Stock (Ts) molarity? M1V1 = M2V2 Ts V1 = 0.05M(100) Solve for V1. Bring up remaining volume with water, to 100 ml.
For 100 ml, add 2 ml of 5 M NaCl, 5 ml of 1 M Tris-HCl stock at the appropriate pH, 2 ml 0.5 M EDTA and Q.S. with distilled water. Autoclave.
Created on 1/30/01 4: 17 PM Tris·Cl [tris(hydroxymethyl) amino methane] , 1 M ... pH ~7.2 or above pH ~9.0. dNTP dilution from Boehringer Mannheim To make an 800l stock ...
Take a small amount of tris-saturated phenol from the stock. Add equal volume of chloroform. It is convenient to prepare it in a 50mL falcon tube.
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